Water deficit stress effects on reactive oxygen metabolism in Catharanthus roseus; impacts on ajmalicine accumulation

C. Abdul Jaleel, B. Sankar, P. V. Murali, M. Gomathinayagam, G. M.A. Lakshmanan, R. Panneerselvam

Research output: Contribution to journalArticlepeer-review

93 Citations (Scopus)

Abstract

In the present work, we have analysed the changes in the reactive oxygen metabolism of Catharanthus roseus (L.) G. Don. plants in terms of H2O2 content, lipid peroxidation and the free radical quenching systems (non-enzymatic and enzymatic antioxidants) under drought stress. In addition to this, the root alkaloid ajmalicine was extracted and quantified from both control and drought stressed plants. The H2O2 content was analysed from both stressed and unstressed control plants. Lipid peroxidation was estimated as thiobarbituric acid reactive substances. The non-enzymatic antioxidants viz., ascorbic acid, α-tocopherol and reduced glutathione contents, antioxidant enzymes like superoxide dismutase, ascorbate peroxidase, and catalase were extracted and estimated from the samples. The alkaloid ajmalicine was extracted and quantified from shade dried root samples and found significantly increased over control. From the results of this investigation, it can be concluded that the water deficit areas may be well used for the cultivation of medicinal plants like C. roseus and the economically important alkaloid production can be enhanced in the plant level.

Original languageEnglish
Pages (from-to)105-111
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume62
Issue number1
DOIs
Publication statusPublished - Mar 15 2008
Externally publishedYes

Keywords

  • Ajmalicine
  • Antioxidants
  • Catharanthus roseus
  • Drought

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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