Viability of escherichia coli o157:H7 during fermentation and storage of plain and spiced ayran

Amin N. Olaimat, Mohammed Belal, Hatim Abulola, Raed Abu Odeh, Amita Attlee, Anas A. Al-Nabulsi, Tareq M. Osaili, Islam Al-Shami, Mutamed M. Ayyash, Reyad Shaker Obaid

Research output: Contribution to journalArticlepeer-review

Abstract

Ayran is a traditional fermented liquid milk product that contains high content of vitamins and minerals and it can be industrially or homemade prepared, therefore, the potential risk of bacterial contamination is high. The objective of the current study was to study the behavior of Escherichia coli O157:H7 during processing and storage of Ayran manufactured with different spices. Pasteurized milk was inoculated with a 5.7 log10 CFU/mL of a 4 strains E. coli O157:H7 cocktail and thermophilic yogurt lactic starter culture. Spices powders including ginger, green tea and fenugreek were separately added to inoculated milk at a concentration of 1% (wt/v). The inoculated milk was fermented at 42 0C for 5 h, and then the samples were cooled and subsequently stored at 4 0C for 5 days. A 20μl aliquot of milk or Ayran samples and their appropriate serial dilutions (0.1% peptone water) were plated in triplicate on eosin methylene blue (EMB) agar for E. coli O157:H7 or De Man, Rogosa and Sharpe agar (MRS) for lactic acid bacteria (LAB). Differences among treatments were analyzed by t-test using the Minitab for Windows software (release 12, Minitab, Inc., State College, PA). Significant differences between treatments were concluded when p was < 0.05. Results showed that E. coli O157:H7 numbers in spiced or control Ayran (without spices) increased by 0.8 to 1.5 log10 CFU/mL during the fermentation process and significantly decreased by 2.0 to 2.8 log10 CFU/mL during storage period. In general, green tea and ginger slightly reduced the viability of E. coli O157:H7 in Ayran compared to control. Ability of E. coli O157:H7 to survive in spiced or nonspiced Ayran may increase the risk of foodborne illness; therefore, prevent of contamination and sanitation practices should be applied to minimize the potential risk of E. coli O157:H7 in Ayran.

Original languageEnglish
Pages (from-to)21-25
Number of pages5
JournalJournal of Hygienic Engineering and Design
Volume29
Publication statusPublished - 2019

Keywords

  • Ayran
  • E. coli O157:H7
  • Fermented milk
  • Lactic acid bacteria
  • Spices

ASJC Scopus subject areas

  • Food Science
  • Safety, Risk, Reliability and Quality

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