An integrated assembly sequence planning and fixture layout planning is developed to minimize assembly process variation. The model utilizes design structure matrix (DSM) to re-sequence assembly process by considering product key characteristics (PKC) and assembly key characteristics (AKC). The DSM gives sufficient assembly constraint at minimum number of fixtures. In the next stage, E-optimality criterion is applied to plan fixture layout for 3-D object. The layout maintains assembly process rigidity and stability. The validation shows that the proposed model capable of identifying optimum fixture layout to support assembly sequence planning. The validation results show that minimum Eigen value of E-optimality gives lower assembly variation. Application of AI tools is one of among future research directions that are proposed. Assembly sequence planning and fixture layout planning integration is the result of this article.