Transcriptome profiling of haloxylon persicum (Bunge ex boiss and buhse) an endangered plant species under peg-induced drought stress

Fayas Thayale Purayil, Balaji Rajashekar, Shyam S. Kurup, Abdul Jaleel Cheruth, Sreeramanan Subramaniam, Nadia Hassan Tawfik, Khaled M.A. Amiri

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Haloxylon persicum is an endangered western Asiatic desert plant species, which survives under extreme environmental conditions. In this study, we focused on transcriptome analysis of H. persicum to understand the molecular mechanisms associated with drought tolerance. Two different periods of polyethylene glycol (PEG)-induced drought stress (48 h and 72 h) were imposed on H. persicum under in vitro conditions, which resulted in 18 million reads, subsequently assembled by de novo method with more than 8000 transcripts in each treatment. The N50 values were 1437, 1467, and 1524 for the control sample, 48 h samples, and 72 h samples, respectively. The gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis resulted in enrichment of mitogen-activated protein kinase (MAPK) and plant hormone signal transduction pathways under PEG-induced drought conditions. The differential gene expression analysis (DGEs) revealed significant changes in the expression pattern between the control and the treated samples. The KEGG analysis resulted in mapping transcripts with 138 different pathways reported in plants. The differential expression of drought-responsive transcription factors depicts the possible signaling cascades involved in drought tolerance. The present study provides greater insight into the fundamental transcriptome reprogramming of desert plants under drought.

Original languageEnglish
Article number640
Pages (from-to)1-19
Number of pages19
JournalGenes
Volume11
Issue number6
DOIs
Publication statusPublished - Jun 2020

Keywords

  • De novo transcriptome
  • Drought resistant
  • Haloxylon persicum
  • White saxaul

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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