The RSC Complex Localizes to Coding Sequences to Regulate Pol II and Histone Occupancy

Marla M. Spain, Suraiya A. Ansari, Rakesh Pathak, Michael J. Palumbo, Randall H. Morse, Chhabi K. Govind

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

ATP-dependent chromatin remodelers regulate chromatin structure during multiple stages of transcription. We report that RSC, an essential chromatin remodeler, is recruited to the open reading frames (ORFs) of actively transcribed genes genome wide, suggesting a role for RSC in regulating transcription elongation. Consistent with such a role, Pol II occupancy in the ORFs of weakly transcribed genes is drastically reduced upon depletion of the RSC catalytic subunit Sth1. RSC inactivation also reduced histone H3 occupancy across transcribed regions. Remarkably, the strongest effects on Pol II and H3 occupancy were confined to the genes displaying the greatest RSC ORF enrichment. Additionally, RSC recruitment to the ORF requires the activities of the SAGA and NuA4 HAT complexes and is aided by the activities of the Pol II CTD Ser2 kinases Bur1 and Ctk1. Overall, our findings strongly implicate ORF-associated RSC in governing Pol II function and in maintaining chromatin structure over transcribed regions.

Original languageEnglish
Pages (from-to)653-666
Number of pages14
JournalMolecular Cell
Volume56
Issue number5
DOIs
Publication statusPublished - Dec 4 2014
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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