The protective effect of apigenin on myocardial injury in diabetic rats mediating activation of the PPAR-γ pathway

Umesh B. Mahajan, Govind Chandrayan, Chandragouda R. Patil, Dharamvir Singh Arya, Kapil Suchal, Yogeeta O. Agrawal, Shreesh Ojha, Sameer N. Goyal

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

We substantiated the role of peroxisome proliferator-activated receptor-γ (PPAR-γ) activation in the protective effect of apigenin against the myocardial infarction (MI) in diabetic rats. Diabetes was induced by intraperitoneal administration of a single dose of streptozotocin (55 mg/kg). The study groups included diabetic rats receiving vehicle, apigenin (75 mg/kg/day, orally), GW9662 (1 mg/kg/day, intraperitoneally), and a combination of apigenin and GW9662 for 14 days. The MI was induced in all the study groups except the diabetic control group by subcutaneous injection of 100 mg/kg/day of isoproterenol on the two terminal days. The diabetes and isoproterenol-induced MI was evident as a reduction in the maximal positive and negative rate of developed left ventricular pressure and an increase in the left ventricular end-diastolic pressure. The activities of creatine kinase on myocardial bundle (CK-MB) and lactate dehydrogenase (LDH) were also reduced. Apigenin treatment prevented the hemodynamic perturbations, restored the left ventricular function and reinstated a balanced redox status. It protected rats against an MI by attenuating myonecrosis, edema, cell death, and oxidative stress. GW9662, a PPAR-γ antagonist reversed the myocardial protection conferred by apigenin. Further, an increase in the PPAR-γ expression in the myocardium of the rats receiving apigenin reinforces the role of PPAR-γ pathway activation in the cardioprotective effects of apigenin.

Original languageEnglish
Article number756
JournalInternational journal of molecular sciences
Volume18
Issue number4
DOIs
Publication statusPublished - Apr 2017

Keywords

  • Apigenin
  • Diabetic cardiac complications
  • Myocardial infarction
  • PPAR-γ
  • Streptozotocin

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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