The K-segments of the wheat dehydrin DHN-5 are essential for the protection of lactate dehydrogenase and β-glucosidase activities in vitro

Marwa Drira, Walid Saibi, Faïçal Brini, Ali Gargouri, Khaled Masmoudi, Moez Hanin

Research output: Contribution to journalArticlepeer-review

75 Citations (Scopus)

Abstract

The wheat dehydrin DHN-5 has been previously shown to exhibit heat protecting effect on enzymatic activities. In order to understand the molecular mechanism by which DHN-5 exerts its protective function, we performed an approach to dissect the functional domains of DHN-5 responsible for this feature. In two distinct enzymatic assays, we found that the truncated forms of DHN-5 containing only one K- or two K-segments are able to protect albeit to less extent than the wild type protein, lactate dehydrogenase and β-glucosidase against damage induced by various stresses in vitro. However, the YS- and Φ-segments alone have no protective effects on these enzymes. Therefore, our study provides the evidence that the protective function of DHN-5 seems to be directly linked to its K-segments which through their amphipatic α-helical structure, may act to prevent protein aggregation.

Original languageEnglish
Pages (from-to)643-650
Number of pages8
JournalMolecular Biotechnology
Volume54
Issue number2
DOIs
Publication statusPublished - Jun 2013
Externally publishedYes

Keywords

  • Abiotic stress
  • Dehydrins
  • K-segment
  • Lactate dehydrogenase
  • β-Glucosidase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Fingerprint

Dive into the research topics of 'The K-segments of the wheat dehydrin DHN-5 are essential for the protection of lactate dehydrogenase and β-glucosidase activities in vitro'. Together they form a unique fingerprint.

Cite this