The H3 receptor is involved in cholecystokinin inhibition of food intake in rats

Samir Attoub, Laurent Moizo, Iradj Sobhani, Jean Pierre Laigneau, Miguel J.M. Lewin, André Bado

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

We investigated the peripheral effects of an H3-receptor agonist and an H3-receptor antagonist (R)α-methylhistamine (Rα-MeHA) and thioperamide, respectively, on basal feeding and the CCK8-induced inhibition of food intake in rat. Intraperitoneal injection of thioperamide reduced food intake in a dose-dependent manner with maximal inhibition (35%, P<0.01 vs saline) at 3 mg/kg. (R)α-MeHA (0.3-3 mg/kg i.p.), an H3-receptor agonist alone had no effect on feeding but reversed the thioperamide-induced inhibition of food intake in a dose-dependent manner. The maximal feeding inhibitory dose of thioperamide (3 mg.kg i.p) increased by 40% and 22 % (P<0.01 vs saline) brain and stomach histamine contents, respectively. Histamine (0.3 - 6 mg/kg i.p.) and CCK-8 (3 - 30 μg/kg i.p) also inhibited food intake in a dose-dependent manner. Inhibition was 20% to 40% for histamine and 40% to 80% (P<0.01 vs saline) for CCK8. CCK-8 inhibition of feeding was increased by thioperamide and prevented by (R)α-MeHA in a dose-dependent way. In addition, CCK-8 did not reduce food intake if rats were pretreated with pyrilamine or ranitidine postsynaptic H1- and H2-receptor antagonists respectively. Our data suggest that the H3-receptor is involved in basal feeding. They also suggest that CCK satiety depends upon the release of histamine which acts on the H2- and H1-receptors, the final mediators of this effect.

Original languageEnglish
Pages (from-to)469-478
Number of pages10
JournalLife Sciences
Volume69
Issue number4
DOIs
Publication statusPublished - Jun 15 2001
Externally publishedYes

Keywords

  • CCK
  • H-receptor
  • Histamine
  • Satiety

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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