The endocrine cells in the gastroenteropancreatic system of the bowfin, Amia calva L. An immunohistochemical, ultrastructural, and immunocytochemical analysis

John H. Youson, Azza A. Al-Mahrouki, Diana Naumovski, J. Michael Conlon

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The gastroenteropancreatic (GEP) endocrine system of bowfin (Amia calva) was described using light and electron microscopy and immunological methods. The islet organ (endocrine pancreas) consists of diffusely scattered, mostly small islets and isolated patches of cells among and within the exocrine acini. The islets are composed of abundant, centrally located B cells immunoreactive to bovine and lamprey insulin antisera and D cells showing a widespread distribution and specificity to somatostatin antibodies. A and F cells are present at the very periphery of the islets and are immunoreactive with antisera against glucagon (and glucagon-like peptide) and several peptides of the pancreatic polypeptide (PP)-family, respectively. The peptides of the two families usually collocates within the same peripheral islet cells and are the most common immunoreactive peptides present in the extra-islet tissue. Immunocytochemistry and fine structural observations characterised the granule morphology for B and D cells and identified two cell types with granules immunoreactive to glucagon antisera. These two putative A cells had similar granules, which were distinct from either B or D cells, but one of the cells had rodshaped cytoplasmic inclusions within cisternae of what appeared to be rough endoplasmic reticulum. The inclusions were not immunoreactive to either insulin or glucagon antisera. Only small numbers of cells in the stomach and intestine immunoreacted to antisera against somatostatin, glucagon, and PP-family peptides. The paucity of these cells was reflected in the low concentrations of these peptides in intestinal extracts. The GEP system of bowfin is not unlike that of other actinopterygian fishes, but there are some marked differences that may reflect the antiquity of this system and/or may be a consequence of the ontogeny of this system in this species.

Original languageEnglish
Pages (from-to)208-224
Number of pages17
JournalJournal of Morphology
Volume250
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Bowfin immunohistochemistry
  • Endocrine
  • GEP
  • Pancreas
  • Ultrastructure

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

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