The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats

Diaa E.E. Rizk, Mohamed A. Fahim, Hazem A. Hassan, Ahmed H. Al-Marzouqi, Gaber A. Ramadan, Soha S. Al-Kedrah, Layla S. Al-Ghafri

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The aim of this study was to compare the effects of ageing and ovariectomy on biomarkers of urogenital ageing in old and young-adult rats. Fisher 344 rats (18- and 3-months-old, n = 6 × 2) underwent ovariectomy. Age-matched sham animals received no intervention (n = 6 × 2). One month later, biomarkers of urogenital ageing were evaluated (light microscopic count of urethral and anal canal submucosal blood vessels, Western blot analysis of urethral, and anal canal submucosal collagen I and III and cytoplasmic p27kip1 expression in the striated urethral and anal sphincters and levator ani and gel electrophoresis of isomyosin I proportion in these muscles) and compared in all groups (n = 24). All biomarkers of urogenital ageing studied were significantly increased in old compared to young-adult sham rats. Ovariectomy significantly increased these changes further in old versus young-adult rats with either smaller or larger differential effect than ageing compared to young-adult sham animals. Ovariectomy significantly exacerbates normative urogenital ageing changes in rats.

Original languageEnglish
Pages (from-to)1077-1085
Number of pages9
JournalInternational Urogynecology Journal
Volume18
Issue number9
DOIs
Publication statusPublished - Sep 2007

Keywords

  • Ageing
  • Incontinence
  • Menopause
  • Rat

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Urology

Fingerprint

Dive into the research topics of 'The effect of ovariectomy on biomarkers of urogenital ageing in old versus young adult rats'. Together they form a unique fingerprint.

Cite this