The constitutively active V2 receptor mutants conferring NSIAD are weakly sensitive to agonist and antagonist regulation

Julie Tenenbaum, Mohammed A. Ayoub, Sanja Perkovska, Anne Laure Adra-Delenne, Christiane Mendre, Bruno Ranchin, Giamperro Bricca, Ghislaine Geelen, Bernard Mouillac, Thierry Durroux, Denis Morin

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Patients having the nephrogenic syndrome of inappropriate antidiuresis present either the R137C or R137L V2 mutated receptor. While the clinical features have been characterized, the molecular mechanisms of functioning of these two mutants remain elusive. In the present study, we compare the pharmacological properties of R137C and R137L mutants with the wild-type and the V2 D136A receptor, the latter being reported as a highly constitutively active receptor. We have performed binding studies, second messenger measurements and BRET experiments in order to evaluate the affinities of the ligands, their agonist and antagonist properties and the ability of the receptors to recruit b-arrestins, respectively. The R137C and R137L receptors exhibit small constitutive activities regarding the Gs protein activation. In addition, these two mutants induce a constitutive b-arrestin recruitment. Of interest, they also exhibit weak sensitivities to agonist and to inverse agonist in term of Gs protein coupling and β-arrestin recruitment. The small constitutive activities of the mutants and the weak regulation of their functioning by agonist suggest a poor ability of the antidiuretic function to be adapted to the external stimuli, giving to the environmental factors an importance which can explain some of the phenotypic variability in patients having NSIAD.

Original languageEnglish
Article numbere8383
JournalPLoS ONE
Volume4
Issue number12
DOIs
Publication statusPublished - 2009
Externally publishedYes

ASJC Scopus subject areas

  • General

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