Synthesis and release of acetylcholine by the isolated perifused trout caudal neurosecretory system

J. Michael Conlon, Richard J. Balment

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The neurons of the caudal neurosecretory system of teleosts contain, in addition to urotensin I and urotensin II, a high concentration of acetylcholine (T. Ichikawa, 1978, Gen. Comp. Endocrinol. 35, 226-233). The isolated urophysis (and attached terminal spinal cord region) of the rainbow trout Oncorhynchus mykiss was incubated with [3H]choline (0.2 MBq/ml) for 45 min at 22° in the presence of the cholinesterase inhibitor, physostigmine. Unreacted choline was removed by perifusion with fish Ringer solution. Incorporation of radioactivity into newly synthesized [3H]acetylcholine was 4.9 ± 2.1 x 105 Bq/g wet tissue wt. When incubations were carried out in the presence of hemicholinium-3, an inhibitor of high-affinity choline uptake, or when physostigmine was omitted from the incubation buffer and/or when [3H]-inulin was substituted for [3H]choline, the incorporation of radioactivity was greatly reduced (<0.5 x 105 Bq/g). The release of [3H]acetylcholine from the preparation increased to 338 ± 59% of basal (P < 0.05) when the concentration of K+ in the perifusion buffer was raised to 41 mM, but neither urotensin I (10-7 M) nor urotensin II (10-6 M) had a significant effect on release. The data indicate that the trout caudal neurosecretory system possesses a high-affinity uptake system for choline and that newly synthesized acetylcholine is released in response to a depolarizing stimulus.

Original languageEnglish
Pages (from-to)36-40
Number of pages5
JournalGeneral and Comparative Endocrinology
Volume103
Issue number1
DOIs
Publication statusPublished - Jul 1996
Externally publishedYes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

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