Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion

Ji Kang Fang, Subbuswamy K. Prabu, Naresh B. Sepuri, Haider Raza, Hindupur K. Anandatheerthavarada, Domenico Galati, Joseph Spear, Narayan G. Avadhani

Research output: Contribution to journalArticlepeer-review

73 Citations (Scopus)

Abstract

We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.

Original languageEnglish
Pages (from-to)1302-1310
Number of pages9
JournalFEBS Letters
Volume581
Issue number7
DOIs
Publication statusPublished - Apr 3 2007
Externally publishedYes

Keywords

  • Cytochrome c oxidase
  • Myocardial ischemia/reperfusion
  • Nano-LC-MS/MS analysis
  • Protein kinase A
  • Subunit phosphorylation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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