Sesamin increases alpha-linolenic acid conversion to docosahexaenoic acid in atlantic salmon (Salmo salar L.) hepatocytes: Role of altered gene expression

Sofia Trattner, B. Ruyter, T. K. Østbye, T. Gjøen, V. Zlabek, A. Kamal-Eldin, J. Pickova

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

In vitro cultivated Atlantic salmon (Salmo salar L.), hepatocytes were incubated without or with a mixture of sesamin and episesamin in order to test for possible effects on lipid metabolism. Sesamin/episesamin exposure (0.05 mM, final concentration) led to increased elongation and desaturation of 14C 18:3n-3 to docosahexaenoic acid (14C 22:6n-3, DHA, P < 0.01) and down regulated gene expression of Δ6 and Δ5 desaturases compared to control treatment. Sesamin/episesamin further increased the hepatocytes capacity for fatty acid β-oxidation of 14C 18:3n-3 (P < 0.01) to the 14C acid soluble products, acetate, malate and oxaloacetate, in agreement with an increased gene expression of carnitine palmitoyltransferase I. Also the gene expression of cluster of differentiation 36 was upregulated and the expression of scavenger receptor type B, peroxisome proliferator-activated receptors α and γ were downregulated. The amount of triacylglycerols secreted by the cells tended to be lower in the sesamin/episesamin incubated hepatocytes than the control cells. This study shows that sesamin has favourable effects on lipid metabolism leading to increased level of DHA, which may be of interest for aquaculture use.

Original languageEnglish
Pages (from-to)999-1008
Number of pages10
JournalLipids
Volume43
Issue number11
DOIs
Publication statusPublished - Nov 2008
Externally publishedYes

Keywords

  • Desaturation
  • Docosahexaenoic acid
  • Hepatocytes
  • Sesamin
  • α-Linolenic acid

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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