Sequences intervening between the core packaging determinants are dispensable for maintaining the packaging potential and propagation of feline immunodeficiency virus transfer vector RNAs

Farah Mustafa, Akela Ghazawi, Preethi Jayanth, Pretty Susan Phillip, Jahabar Ali, Tahir A. Rizvi

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

The packaging determinants of feline immunodeficiency virus (FIV) consist of two discontinuous core regions, extending from R to ∼150 bp of the 5′ untranslated region and the first ∼100 bp of gag. However, the role of sequences intervening between the core regions in packaging has not been clear. A mutational analysis was conducted to determine whether the intervening sequences played a role in FIV RNA packaging, using an in vivo packaging assay complemented with semiquantitative reverse transcriptase PCR. Our analyses reveal that the intervening sequences are dispensable not only for vector RNA packaging but also for propagation, confirming the discontinuous nature of the FIV packaging signal.

Original languageEnglish
Pages (from-to)13817-13821
Number of pages5
JournalJournal of Virology
Volume79
Issue number21
DOIs
Publication statusPublished - Nov 2005

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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