SAP30, a novel protein conserved between human and yeast, is a component of a histone deacetylase complex

Yi Zhang, Zu Wen Sun, Rabah Iratni, Hediye Erdjument-Bromage, Paul Tempst, Michael Hampsey, Danny Reinberg

Research output: Contribution to journalArticlepeer-review

237 Citations (Scopus)

Abstract

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Recently, histone acetylation and deacetylation were found to be catalyzed by structurally distinct, multisubunit complexes that mediate, respectively, activation and repression of transcription. Here, we identify SAP30 as a novel component of the human histone deacetylase complex that includes Sin3, the histone deacetylases HDAC1 and HDAC2, histone binding proteins RbAp46 and RbAp48, as well as other polypeptides. Moreover, we describe a SAP30 homolog in yeast that is functionally related to Sin3 and the histone deacetylase Rpd3. The human SAP30 complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones due to the inability of the histone binding proteins RbAp46 and RbAp48 to gain access to nucleosomal histones. These results define SAP30 as a component of a histone deacetylase complex conserved among eukaryotic organisms.

Original languageEnglish
Pages (from-to)1021-1031
Number of pages11
JournalMolecular Cell
Volume1
Issue number7
DOIs
Publication statusPublished - Jun 1998
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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