Retained activity of an O25b-specific monoclonal antibody against an Mcr-1-producing Escherichia coli sequence type 131 strain

Luis M. Guachalla, Katharina Ramoni, Cecilia Varga, Michele Mutti, Akela Ghazawi, Tibor Pál, Eszter Nagy, Ágnes Sonnevend, Gábor Nagy, Valéria Szijártó

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Plasmid-encoded colistin resistance is emerging among extraintestinal pathogenic Escherichia coli strains, including those of the epidemic clone sequence type 131 (ST131)-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of lipopolysaccharide (LPS), conferring resistance to polymyxins. We investigated whether this modification changed the activity of the monoclonal antibody ASN-4, specific to the O25b side chain of ST131 LPS. We confirmed that, unlike colistin, ASN-4 retained its bactericidal and endotoxin-neutralizing activities and therefore offers a treatment option against extremely drug-resistant ST131 isolates.

Original languageEnglish
Article numbere00046-18
JournalAntimicrobial Agents and Chemotherapy
Volume62
Issue number7
DOIs
Publication statusPublished - Jul 2018

Keywords

  • Escherichia coli ST131
  • Mcr-1
  • Monoclonal antibodies

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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