Rapid effects of cytochalasin-D on contraction and intracellular calcium in single rat ventricular myocytes

F. C. Howarth, M. R. Boyett, E. White

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Contraction and intracellular calcium ([Ca2+](i)) transients were recorded using a video edge detector and fluorescence spectrophotometry, respectively, in rat ventricular myocytes at 22-24°C stimulated at a frequency of 1 Hz. Application of the F-actin disrupter cytochalasin-D (Cyt-D) caused a large reduction in the amplitude of contraction and a small increase in the [Ca2+](i) transient. These responses began within a few seconds of application and were complete after 2 min of exposure. Phase-plane relationships of contraction and [Ca2+](i) were consistent with cytochalasin-D causing a decrease in myofilament responsiveness to Ca2+.

Original languageEnglish
Pages (from-to)804-806
Number of pages3
JournalPflugers Archiv European Journal of Physiology
Volume436
Issue number5
DOIs
Publication statusPublished - Sep 23 1998
Externally publishedYes

Keywords

  • Cytochalasan-D
  • Excitation-contraction coupling
  • Heart

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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