Radioimmunoassay of neuropeptide Y

J. M. Allen, J. C. Yeats, T. E. Adrian, S. R. Bloom

Research output: Contribution to journalArticlepeer-review

122 Citations (Scopus)

Abstract

The development of a radioimmunoassay to the newly isolated peptide, neuropeptide Y is described. Four separate antisera have been developed using different immunisation schedules. Two of these antisera (YNI and YNIO) are directed to the C-terminal region of the peptide and cross-react with the related peptide PYY, whereas YN7 is specific being directed to the N-terminal region of NPY, YN6 is similarly specific for NPY, but is unable to bind the available fragments. These four antisera provide similar results for determination of NPY immunoreactivity within porcine brain extracts, however YN6 consistently undervalues all extracts from the other species examined (human, rat, guinea pig, cat and mouse). Chromatographic analysis by means of reverse phase high pressure liquid chromatography (HPLC) shows that NPY immunoreactivity of human extracts elutes in an earlier position than the porcine standard. It seems likely therefore that human and porcine NPY differ in their amino acid sequences.

Original languageEnglish
Pages (from-to)61-70
Number of pages10
JournalRegulatory Peptides
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 1984
Externally publishedYes

Keywords

  • NPY fragments
  • pancreatic polypeptides
  • peptide YY
  • reverse phase HPLC
  • species
  • specificity

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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