Proteomic and physiological assessment of stress sensitive and tolerant variety of tomato treated with brassinosteroids and hydrogen peroxide under low-temperature stress

Tanveer Alam Khan, Mohammad Yusuf, Aqeel Ahmad, Zoobia Bashir, Taiba Saeed, Qazi Fariduddin, Shamsul Hayat, Hans Peter Mock, Tingquan Wu

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

The aim of current investigation was to perform proteomics and physio-chemical studies to dissect the changes in contrasting varieties (S-22 and PKM-1) of Lycopersicon esculentum under low-temperature stress. Plant grown under variable low-temperature stress were analysed for their growth biomarkers, antioxidant enzyme activities, and other physiological parameters, which headed toward the determination of protein species responding to low-temperature and 24-epibrassinolide (EBL) concentrations. The plants grown under temperatures, 20/14, 12/7, and 10/3 °C recorded significantly lower growth biomarkers, SPAD chlorophyll, net photosynthetic rate and carbonic anhydrase activity in S-22 and PKM-1. Moreover, the combined effect of EBL and hydrogen peroxide (H 2 O 2 ) significantly improved the parameters mentioned above and consecutively upgraded the different antioxidant enzymes (CAT and SOD) with higher accumulation of proline under stress and stress-free environments. Furthermore, proteomics study revealed that the maximum number of differentially expressed proteins were detected in S-22 (EBL + H 2 O 2 ); while treatment with EBL + H 2 O 2 + low temperature lost expression of 20 proteins. Overall, three proteins (O80577, Q9FJQ8, and Q9SKL2) took a substantial part in the biosynthesis of citrate cycle pathway and enhanced the growth and photosynthetic efficiency of tomato plants under low-temperature stress.

Original languageEnglish
Pages (from-to)500-511
Number of pages12
JournalFood Chemistry
Volume289
DOIs
Publication statusPublished - Aug 15 2019

Keywords

  • 2-DE
  • Brassinosteroids
  • Hydrogen peroxide
  • Low temperature
  • Lycopersicon esculentum
  • Proteomics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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