Protein G ELISA for detection of antibodies against Toxoplasma SAG1 in dromedaries.

Yousif A. Abu-Zeid

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

ELISA was used for detection of antibodies against the immunodominant surface antigen 1 (SAG1, synonymous P30) of Toxoplasma gondii, and peroxidase-conjugated protein G was used instead of commercially unavailable enzyme-conjugated anti-dromedary antibody. A latex agglutination test was employed to select 20 seronegative control animals, and peroxidase-conjugated protein A was used for comparison with protein G. The overall seroprevalence rate was 31.4%; males had to some extent higher seropositive rate than females (P = 0.077). Seropositive camels sampled in winter had significantly higher-antibody levels than those sampled in summer (P < 0.01). ELISA values using protein G and protein A conjugates were significantly correlated (Spearman's rho = 0.797; n = 185; P < 0.001). The results were discussed.

Original languageEnglish
Pages (from-to)247-257
Number of pages11
JournalJournal of the Egyptian Society of Parasitology
Volume32
Issue number1
Publication statusPublished - Apr 2002

ASJC Scopus subject areas

  • Medicine(all)

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