Propagation of SIV Vectors by Genetic Complementation with a Heterologous env Gene

Tahir A. Rizvi, Antonito T. Panganiban

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

In order to study SIV replication over a single round of replication virus particles were generated that contain a replication-defective vector containing a selectable marker. Genetic complementation between an env-deficient SIV variant and plasmid that expresses the env gene of an amphotropic murine retrovirus resulted in infectious SIV particles containing the vector. These pseudotyped particles exhibited an expanded host range through the use of an alternative receptor. This system should be useful in the genetic analysis of SIV nucleic acid replication. To determine whether the terminal cis acting components of the SIV genome might be sufficient for viral nucleic acid propagation a vector was generated which lack the internally located rev-responsive element. Propagation of this vector was reduced by at least 100-fold.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 1992
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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