Primary structures and effects on gastrointestinal motility of tachykinins from the rainbow trout

J. Jensen, K. R. Olson, J. M. Conlon

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Purification and structural characterization of tachykinins from rainbow trout (Oncorhynchus mykiss) intestine has demonstrated the presence of three different peptides related to the mammalian tachykinins: substance P, neurokinin A, and neuropeptide-γ. The substance P- and the neurokinin A- related peptides present in the intestine are identical to the tachykinins previously isolated from the trout brain. The neuropeptide-γ-related peptide (Ser-Ser-Ala-Asn-Pro-Gln-Ile-Thr-Arg-Lys-Arg-His-Lys-Ile-Asn-Ser-Phe-Val- Gly-Leu-Met-NH2), not previously identified in brain tissue, has the sequence of the neurokinin A-related tachykinin at its COOH-terminus. Both trout substance P and neurokinin A stimulated the motility of isolated trout intestinal muscle [pD2 (-log of EC50) values 8.5 ± 0.15 and 7.35 ± 0.08, respectively] and the vascularly perfused trout stomach (pD2 values 9.63 ± 0.23 and 8.18 ± 0.23, respectively). Trout substance P was 14 times more potent than trout neurokinin A in the intestine and 28 times more potent in the stomach. The data suggest that receptors interacting with tachykinins in the trout gastrointestinal tract have a similar selectivity as the mammalian NK-1 receptor.

Original languageEnglish
Pages (from-to)R804-R810
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume265
Issue number4 34-4
DOIs
Publication statusPublished - 1993
Externally publishedYes

Keywords

  • fish
  • gastrointestinal motility
  • neurokinin A
  • neuropeptide-γ
  • substance P

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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