Preservation of acetylcholine muscarinic M2 receptor G-protein interactions in the neocortex of patients with Alzheimer's disease

Angel Hérnandez-Hérnandez, Abdu Adem, Rivka Ravid, Richard F. Cowburn

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The efficacy of acetylcholine muscarinic M2 receptor-G-protein coupling was investigated in Alzheimer's disease and control neocortical membranes by measuring the effects of M9Cl2 and 5′-guanylylimidodiphosphate (Gpp[NH]p) on high-affinity [3H]oxotremorine-M ([3H]OXO-M) binding. M9Cl2 gave similar enhancements of [3H]OXO-M binding in Alzheimer's disease and control occipital cortex. In contrast, M9Cl2 enhanced [3H]OXO-M binding was significantly higher in Alzheimer's disease superior temporal cortex, compared to controls. MgCl2 enhanced [3H]OXO-M binding in both the occipital and temporal cortices of the Alzheimer's disease cases was reversed to control levels by Gpp[NH]p. It is concluded that the number of high-affinity muscarinic M2 sites is increased in Alzheimer's disease superior temporal, but not occipital, cortex and that M2 sites in both regions maintain an efficient G-protein coupling.

Original languageEnglish
Pages (from-to)57-60
Number of pages4
JournalNeuroscience Letters
Volume186
Issue number1
DOIs
Publication statusPublished - Feb 15 1995
Externally publishedYes

Keywords

  • Acetylcholine
  • Alzheimer's disease
  • G-protein
  • Muscarinic receptor
  • Post-mortem human brain

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint

Dive into the research topics of 'Preservation of acetylcholine muscarinic M2 receptor G-protein interactions in the neocortex of patients with Alzheimer's disease'. Together they form a unique fingerprint.

Cite this