Physicochemical, bioactive and rheological properties of an exopolysaccharide produced by a probiotic Pediococcus pentosaceus M41

Mutamed Ayyash, Basim Abu-Jdayil, Amin Olaimat, Gennaro Esposito, Pariyaporn Itsaranuwat, Tareq Osaili, Reyad Obaid, Jaleel Kizhakkayil, Shao Quan Liu

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

This study aimed to investigate the physicochemical properties, health-promoting benefits and rheological properties of an EPS produced by a novel probiotic Pediococcus pentosaceus M41 isolated from a marine source. P. pentosaceus M41 was able to produce an EPS with average molecular weight of 682.07 kDa. EPS-M41 consisted of arabinose, mannose, glucose and galactose with a molar ratio of 1.2:1.8:15.1:1.0. EPS-M41 structure could be proposed as →3)α-D-Glc(1→2)β-D-Man(1→2)α-D-Glc(1→6)α-D-Glc(1→4)α-D-Glc(1→4)α-D-Gal(1→ with arabinose linked at the terminals. At concentration of 10 mg.ml−1, the antioxidant capacity was 76.5% and 48.9% for DPPH and ABTS, respectively. EPS-M41 inhibited 86.8% and 90.8% of the α-amylase and α-glucosidase activities, respectively, at 100 μg.ml−1. A 77.5% and 46.4% of antitumor inhibition occurred by EPS-M41 against Caco-2 and MCF-7 cells, respectively. The apparent viscosity (ƞ) of all EPS-M41 solutions decreased with shear rate increases. Salt type and pH value had an impact on the rheological properties of EPS-M41.

Original languageEnglish
Article number115462
JournalCarbohydrate Polymers
Volume229
DOIs
Publication statusPublished - Feb 1 2020

Keywords

  • 1,1-diphenyl-2-picrylhydrazyl DPPH (PubChem CID: 2735032)
  • Antidiabetic
  • Antioxidants
  • Antitumor
  • D-Arabinose (PubChem CID:439195)
  • D-Galactose (PubChem CID:6036)
  • D-Glucose (PubChem CID:5793)
  • Deuterium oxide (PubChem CID:24602)
  • Exopolysaccharides
  • Pediococcus pentosaceus
  • Rheological properties
  • Trifluoroacetic acid (PubChem CID:6422)
  • p-nitrophenyl ⍺-D-glucopyranoside (PubChem CID: 102764)

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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