Peptidomic analysis of the host-defense peptides in skin secretions of the Trinidadian leaf frog Phyllomedusa trinitatis (Phyllomedusidae)

Milena Mechkarska, Laurent Coquet, Jérôme Leprince, Renoir J. Auguste, Thierry Jouenne, Maria Luisa Mangoni, J. Michael Conlon

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Peptidomic analysis (reversed-phase HPLC combined with MALDI-TOF mass spectrometry and automated Edman degradation) of norepinephrine-stimulated skin secretions from the Trinidadian leaf frog Phyllomedusa trinitatis Mertens 1926 led to the identification and structural characterization of 26 host-defense peptides. On the basis of amino acid sequence similarity, the peptides may be divided into the followings groups: dermaseptins with the conserved N-terminal region GLWSKIK (6 peptides), dermaseptins with the N-terminal region ALWKXXLK (5 peptides), dermaseptins with the conserved N-terminal region GLFKTLIKGAGKMLGHVAK (4 peptides), C-terminally α-amidated and non-amidated forms of the phylloseptins (9 peptides), phyllocaerulein, a peptide (GLVSGLLNSVTGLLGNLAGGGL) with structural similarity to the plasticins, and a putative antioxidant peptide (LTWKIPTRFCGVT). The primary structures of the peptides support the claim based upon morphological criteria that P. trinitatis and Phyllomedusa tarsius are very closely related phylogenetically but are probably not conspecific. Among the phylloseptins, phylloseptin-1.1TR (FLSLIPKIAGGIASLVKNL.NH2) displayed the most potent antimicrobial activity.

Original languageEnglish
Pages (from-to)72-79
Number of pages8
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume28
DOIs
Publication statusPublished - Dec 2018
Externally publishedYes

Keywords

  • Antioxidant peptide
  • Dermaseptin
  • Frog skin
  • Host-defense peptide
  • Phyllomedusa
  • Phylloseptin
  • Plasticin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics

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