Pax7 reveals a greater frequency and concentration of satellite cells at the ends of growing skeletal muscle fibers

Mohammed Z. Allouh, Zipora Yablonka-Reuveni, Benjamin W.C. Rosser

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

The main sites of longitudinal growth in skeletal muscle are the ends of the fibers. This study tests the hypothesis that satellite cells (SCs) are at a greater frequency (#SC nuclei/all nuclei within basal laminae) and concentration (closer together) within growing fiber ends of posthatch chicken pectoralis. SCs were localized by their Pax7 expression, and fiber ends were identified by their retention of neonatal myosin heavy chains and small cross-sectional profiles. Whereas SC frequency decreased from about 20% at 9 days posthatch to <5% at 115 days, fiber ends retained a frequency of ∼16%. Calculated mean area of sarcolemma per SC revealed higher concentrations of SCs at fiber ends. There was also a strong inverse correlation between SC frequency and fiber profile cross-sectional size throughout development. This study suggests that SCs at fiber ends play a key role in the longitudinal growth of muscle fibers, and that fiber profile size may impact SC distribution.

Original languageEnglish
Pages (from-to)77-87
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume56
Issue number1
DOIs
Publication statusPublished - Jan 2008
Externally publishedYes

Keywords

  • Fiber ends
  • Muscle fibers
  • Muscle growth
  • Myonuclei
  • Myosin
  • Pax7
  • Satellite cells

ASJC Scopus subject areas

  • Anatomy
  • Histology

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