Passage of intratracheally instilled ultrafine particles from the lung into the systemic circulation in hamster

A. Nemmar, H. Vanbilloen, M. F. Hoylaerts, P. H.M. Hoet, A. Verbruggen, B. Nemery

Research output: Contribution to journalArticlepeer-review

512 Citations (Scopus)

Abstract

The mechanisms of particulate pollution-related cardiovascular morbidity and mortality are not well understood. We studied the passage of radioactively labeled ultrafine particles after their intratracheal instillation. Hamsters received a single intratracheal instillation of 100 μg albumin nanocolloid particles (nominal diameter ≤ 80 nm) labeled with 100 μCi technetium-99m and were killed after 5, 15, 30, and 60 min. In blood, radioactivity, expressed as percentage of total body radioactivity per gram blood, amounted to 2.88 ± 0.80%, 1.30 ± 0.17%, 1.52 ± 0.46%, and 0.21 ± 0.06% at 5, 15, 30, and 60 min, respectively. Thin-layer chromatography showed only one peak of radioactivity corresponding to unaltered 99mTc-albumin nanocolloid. In the liver, radioactivity, expressed as percentage of total radioactivity per organ, amounted to 0.10 ± 0.07%, 0.23 ± 0.06%, 1.24 + 0.27%, and 0.06 ± 0.02% at 5, 15, 30, and 60 min, respectively. Lower values were observed in the heart, spleen, kidneys, and brain. Dose dependence was assessed at 30 rain following instillation of 10 μg and 1 μg 99mTc-albumin per animal (n = 3 at each dose), and values of the same relative magnitudes as after instillation of 100 μg were obtained. We conclude that a significant fraction of 99mTc-albumin, taken as a model of ultrafine particles, rapidly diffuses from the lungs into the systemic circulation.

Original languageEnglish
Pages (from-to)1665-1668
Number of pages4
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume164
Issue number9
DOIs
Publication statusPublished - Nov 1 2001
Externally publishedYes

Keywords

  • Air pollution
  • Toxic inhalation

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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