Pancreastatin: A novel peptide inhibitor of parietal cell secretion

Jonathan J. Lewis, Michael J. Zdon, Thomas E. Adrian, Irvin M. Modlin

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Pancreastatin is a recently identified 49-amino-acid peptide found in gastrointestinal tract and gastric mucosa. Its biologic effects on gastric function are unknown. The aim of this study was to determine the effects of pancreastatin [33-49] (the synthetic C-terminal fragment) on acid secretion and somatostatin release in vitro. Isolated rabbit gastric glands were prepared by means of collagenase digestion. Acid secretion was assessed indirectly with use of 14C-aminopyrine (AP) uptake by glands, and somatostatin release from D cells was measured with radioimmunoassay. Pancreastatin alone (10-10 to 10-6 mol/L) had no effect on 14C-AP uptake compared with unstimulated glands. In contrast, pancreastatin inhibited with histamine-(10-6, 10-5 mol/L; p < 0.005) and carbachol-(10-5, 10-4 mol/L; p < 0.001) stimulated 14C-AP uptake in a dose-dependent manner. Neither forskolin-(10-6, 10-4 mol/L; p > 0.50) or 8-Br-cAMP-(10-5, 10-4 mol/L; p > 0.30) stimulated 14C-AP uptake were influenced by pancreastatin. Pancreastatin had no effect on somatostatin release from glands. These data suggest that pancreastatin probably acts at receptor or membrane level, inhibiting both histamine- and carbachol-stimulated 14C-AP uptake. These effects are not mediated by D cell somatostatin release. It is possible that pancreastatin acts as a paracrine or endocrine inhibitory regulator of parietal cell secretion.

Original languageEnglish
Pages (from-to)1031-1036
Number of pages6
JournalSurgery
Volume104
Issue number6
Publication statusPublished - Dec 1988
Externally publishedYes

ASJC Scopus subject areas

  • Surgery

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