Padlock probes: Circularizing oligonucleotides for localized DNA detection

Mats Nilsson, Helena Malmgren, Martina Samiotaki, Marek Kwiatkowski, Bhanu P. Chowdhary, Ulf Landegren

Research output: Contribution to journalArticlepeer-review

598 Citations (Scopus)

Abstract

Nucleotide sequence information derived from DNA segments of the human and other genomes is accumulating rapidly. However, it frequently proves difficult to use such short DNA segments to identify clones in genomic libraries or fragments in blots of the whole genome or for in situ analysis of chromosomes. Oligonucleotide probes, consisting of two target-complementary segments, connected by a linker sequence, were designed. Upon recognition of the specific nucleic acid molecule the ends of the probes were joined through the action of a ligase, creating circular DNA molecules catenated to the target sequence. These probes thus provide highly specific detection with minimal background.

Original languageEnglish
Pages (from-to)2085-2088
Number of pages4
JournalScience
Volume265
Issue number5181
DOIs
Publication statusPublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • General

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