Neurokinin B in a human pheochromocytoma measured with a specific radioimmunoassay

Reinhard Kage, J. Michael Conlon

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

An N-terminally directed antiserum to neurokinin B was raised in rabbits using an immunogen prepared by coupling the free-SH group of neurokinin B extended from its C-terminus by a cysteine residue (NKB-Cys) to an -NH2 group on human serum albumin using a heterobifunctional cross-linking reagent. In radioimmunoassay with 125I-Bolton-Hunter-labelled NKB-Cys as tracer, the antiserum showed no cross-reactivity with other tachykinins. An extract of a human pheochromocytoma, previously shown to contain peptides derived from preprotachykinin A, contained NKB-LI (13 pmol/g wet weight). The retention time of tumor neurokinin B on reversed-phase HPLC was the same as that of synthetic neurokinin B. Peptides with the retention times of substance P, neurokinin A, neurokinin A(3-10)-peptide and neuropeptide K were also identified in the tumor extract. NKB-LI was not detected in extracts of a further nine pheochromocytomas or in five carcinoid tumors that expressed the preprotachykinin A gene.

Original languageEnglish
Pages (from-to)713-716
Number of pages4
JournalPeptides
Volume10
Issue number3
DOIs
Publication statusPublished - 1989
Externally publishedYes

Keywords

  • Neurokinin A
  • Neurokinin B
  • Pheochromocytoma
  • Preprotachykinin
  • Substance P

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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