Mechanism of orexin B-stimulated insulin and glucagon release from the pancreas of normal and diabetic rats

Ernest Adeghate, Rasheed Hameed

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Objectives: To examine the pattern of distribution and effect of orexin B in the islets of normal and diabetic rats. Methods: Pancreatic tissue fragments collected from normal and diabetic (4 weeks after the onset of diabetes) rats were either processed for immunohistochemistry or treated with different concentrations (10 to 10 mol/L) of orexin B. Results: Orexin B-positive nerves were observed in the wall of blood vessels of both normal and diabetic rat pancreas. Orexin B is abundant in the islets of normal rats and colocalized with insulin in β cells. The number of orexin B-positive cells decreased after the onset of diabetes. Orexin B evoked significant (P < 0.05) increases in insulin release from the pancreas of normal and diabetic rats. Propranolol, a β-adrenergic receptor antagonist, significantly (P < 0.04) reduced the stimulatory effect of orexin B on insulin secretion. Orexin B also induced significant (P < 0.05) increases in glucagon release from the pancreas of normal rats but failed to stimulate glucagon secretion from the pancreas of diabetic rats. Conclusions: Orexin B stimulated insulin secretion in normal and diabetic rat pancreas through the β-adrenergic pathway. Orexin B may have an important role in the regulation of islet function.

Original languageEnglish
Pages (from-to)131-136
Number of pages6
JournalPancreas
Volume40
Issue number1
DOIs
Publication statusPublished - Jan 2011

Keywords

  • diabetes
  • hormone
  • immunohistochemistry
  • islet
  • orexin
  • radioimmunoassay

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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