Mannosylated linear and cyclic single amino acid mutant peptides using a small 10 amino acid linker constitute promising candidates against multiple sclerosis

Stephanie Day, Theodore Tselios, Maria Eleni Androutsou, Anthi Tapeinou, Irene Frilligou, Lily Stojanovska, John Matsoukas, Vasso Apostolopoulos

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Multiple sclerosis (MS) is a serious autoimmune demyelinating disease leading to loss of neurological function. The design and synthesis of various altered peptide ligands of immunodominant epitopes of myelin proteins to alter the autoimmune response, is a promising therapeutic approach for MS. In this study, linear and cyclic peptide analogs based on the myelin basic protein 83-99 (MBP83-99) immunodominant epitope conjugated to reduced mannan via the (KG)5 and keyhole limpet hemocyanin (KLH) bridge, respectively, were evaluated for their biological/immunological profiles in SJL/J mice. Of all the peptide analogs tested, linear MBP83-99(F91) and linear MBP83-99(Y91) conjugated to reduced mannan via a (KG)5 linker and cyclic MBP83-99(F91) conjugated to reduce mannan via KLH linker, yielded the best immunological profile and constitute novel candidates for further immunotherapeutic studies against MS in animal models and in human clinical trials.

Original languageEnglish
Article number136
JournalFrontiers in immunology
Volume6
Issue numberAPR
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • Altered peptide ligands
  • MBP83-99
  • Mannan
  • Multiple sclerosis
  • Myelin basic protein

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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