MALDI mass spectrometry analysis of high molecular weight proteins from whole bacterial cells: Pretreatment of samples with surfactants

Mohammed A. Meetani, Kent J. Voorhees

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

The use of surfactants as additives in conjunction with on-probe whole cell bacterial protein analysis employing MALDI-TOF-MS is described. Nonionic and zwitterionic surfactants were used to enhance the detection of high molecular weight proteins. Three nonionic, N-octyl-B-D-glactopyranoside, N-decyl-B-D-maltopyranoside, and N-dodecyl-B-D-maltoside, and two zwitterionic surfactants, N,N-dimethyldodecylamine-N-oxide and zwittergent 3-12 were evaluated with five different MALDI matrix systems. New peaks in the mass range of 2 to 80 kDa were produced with all of the various combinations of matrix and surfactant from both whole cell gram-positive and gram-negative bacteria. Ferulic acid used in conjunction with a 1.0 mM solution of N-octyl-B-D- glactopyranoside produced the highest quality spectra with high signal to noise ratios and peaks up to 140 kDa.

Original languageEnglish
Pages (from-to)1422-1426
Number of pages5
JournalJournal of the American Society for Mass Spectrometry
Volume16
Issue number9
DOIs
Publication statusPublished - Sep 2005
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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