Loss of dystrophin staining in cardiomyocytes: A novel method for detection early myocardial infarction

Satwat Hashmi, Suhail Al-Salam

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Myocardial infarction (MI) is the most frequent diagnosis made in majority of sudden death cases subjected to clinical and medicolegal autopsies. When sudden death occurs at a very early stage of MI, traditional macroscopic examination, or histological stains cannot easily detect the myocardial changes. For this reason we propose a new method for detecting MI at an early stage. Murine model of MI was used to induce MI through permanent ligation of left anterior descending branch of left coronary artery. Five groups of C57B6/J mice were used for inducing MI, which includes 20 minutes, 30 minutes, one hour, four hours and 24 hours post MI groups. One naïve group and sham-operated groups were used as controls. There is loss of dystrophin membranous staining in cardiac myocytes occurs as early as 20 minutes post myocardial infarction. This can be used as a novel method to diagnose early myocardial infarction in post mortem cases where diagnosis is unclear. In conclusion, evaluation of immunohistochemical expression of dystrophin represents a highly sensitive method for detecting early myocardial infarction due to the loss of staining in the infarcted areas. Dystrophin immunostaining can also be used to assess myocardial architecture.

Original languageEnglish
Pages (from-to)249-257
Number of pages9
JournalInternational Journal of Clinical and Experimental Pathology
Volume6
Issue number2
Publication statusPublished - Aug 30 2013

Keywords

  • Dystrophin
  • Early detection
  • Heart
  • Ischemia
  • Myocardial infarction
  • Myocardium

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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