Kinetics of Aspergillus niger cellulase inhibition by reducing sugar produced by the hydrolysis of carboxymethylcellulose

Sulaiman Al-Zuhair, Ling Fan Yu, Soon Chui Kit, Sarmad Rizwan

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Hydrolysis of two types of cellulose, namely, totally amorphous Carboxymethyl-cellulose (CMC) and highly crystalline wood shavings, by Aspergillus niger cellulase was studied in a stirred batch reactor at 50°C. Two kinetic models were devised to predict the rate of reducing sugar production assuming competitive and non-competitive product inhibition of the enzyme. The applicability of the models was tested by comparing experimental results with those predicted by the models. Both models followed the experimental data fairly well, giving standard deviation of 13.3 and 7.9 for the competitive and the non-competitive inhibition models, respectively. However the non-competitive inhibition model showed more realistic behaviour than the non-competitive model and represented better the experimental data. The significance of crystallinity was determined by comparing the production of reducing sugar from wood shavings to the production from CMC. At same initial substrate concentration, it was found that the production of reducing sugar ceased at much shorter times for wood shavings in comparison to that for CMC.

Original languageEnglish
Article numberA7
JournalInternational Journal of Chemical Reactor Engineering
Volume5
DOIs
Publication statusPublished - 2007

Keywords

  • Cellulase
  • Cellulose
  • Enzymatic hydrolysis
  • Product inhibition

ASJC Scopus subject areas

  • Chemical Engineering(all)

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