Ketoconazole-induced testicular damage in rats reduced by Gentiana extract

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32 Citations (Scopus)

Abstract

Ketoconazole (KET) is an antifungal drug with a broad spectrum of activity that also induces reproductive toxicity in humans and animals. The protective effect of Gentiana (GEN) extract (Gentiana lutea) against KET-induced testicular damage was evaluated in male Wistar rats. GEN extract was administered orally (1 g/kg b wt/day) for 26 days. Three weeks after extract administration, KET was co-administered intraperitoneally at a dose of 100 mg/kg once a day for 5 days. KET-induced reproductive toxicity was associated with clear reductions of the weights of testes and epididymides, sperm indices and serum testosterone levels. KET also induced severe testicular histopathological lesions such as degeneration of the seminiferous tubules and depletion of germ cells. In addition, marked oxidative damage to testicular lipids and alterations of natural antioxidants (catalase (CAT) and superoxide dismutase (SOD)) were reported in association with KET toxicity. Most of the KET-induced effects were greatly decreased with the concomitant application of GEN extract. This study suggests a protective role of GEN extract that could be attributed to its antioxidant properties.

Original languageEnglish
Pages (from-to)377-384
Number of pages8
JournalExperimental and Toxicologic Pathology
Volume59
Issue number6
DOIs
Publication statusPublished - Apr 3 2008

Keywords

  • Antioxidant properties
  • Gentiana
  • Ketoconazole
  • Testicular damage

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Cell Biology

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