Isolation and structural characterization of two novel peptides derived from proopiomelanocortin in the pituitary of the rainbow trout

Hélène Tollemer, Jérôme Leprince, Ludovic Galas, Frans Vandesande, Jean Claude Le Mevel, Marie Christine Tonon, J. Michael Conlon, Hubert Vaudry

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The trout possesses two POMC genes as a result of duplication of its genome some 25-100 million years ago. One of the POMC molecules exhibits a unique C-terminal extension of 25 amino acid residues which is not found in any other POMC characterized so far. In order to isolate possible novel peptides derived from trout POMC-A, we have raised antibodies against two synthetic epitopes derived from the C-terminal region of the precursor. Two native decapeptides were isolated in pure form from an extract of trout pituitary. The primary structures of these peptides were established as Glu-Gln-Trp-Gly-Arg-Glu-Glu-Gly-Glu-Glu and Ala-Leu-Gly-Glu-Arg-Lys-Tyr-His-Phe-Gln-NH2. The structure of the trout POMC-A cDNA reveals that both peptides are flanked by pairs of basic amino acids or a glycine residue, indicating that they can actually be generated during post-translational processing of POMC-A.

Original languageEnglish
Pages (from-to)653-657
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume238
Issue number2
DOIs
Publication statusPublished - Sep 18 1997
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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