Isolation and structural characterization of calcitonin gene-related peptide from the brain and intestine of the frog, Rana ridibunda

J. Michael Conlon, Marie Christine Tonon, Hubert Vaudry

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Calcitonin gene-related peptide (CGRP) was isolated in a single molecular form from an extract of the whole brain and from the small intestine of the European green frog, Rana ridibunda. The primary structure of the peptide was established as: Ala-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr- His10-Arg-Leu-Ala-Asp-Phe-Leu-Ser-Arg-Ser-Gly20-Gly-Met-Ala-Lys-Asn-Asn-Phe-Val-Pro-Thr30-Asn-Val-Gly-Ser-Lys-Ala-Phe-NH2. Frog CGRP shows only two amino acid substitutions (Val22 → Met and Gly23 å Ala) compared with chicken CGRP, as deduced from the nucleotide sequence of a cloned cDNA. The Asp14 residue, which leads to enhanced biological potency in chicken CGRP, is also present in the frog peptide. The data show that the structure of CGRP has been strongly conserved during evolution of the vertebrates.

Original languageEnglish
Pages (from-to)581-586
Number of pages6
JournalPeptides
Volume14
Issue number3
DOIs
Publication statusPublished - Jan 1 1993
Externally publishedYes

Keywords

  • Brain
  • Calcitonin gene-related peptide
  • Frog
  • Intestine

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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