Isolation and identification of Brucella melitensis using bacteriological and molecular tools from aborted goats in the Afar region of north-eastern Ethiopia

Muluken Tekle, Mengistu Legesse, Bedaso Mammo Edao, Gobena Ameni, Gezahegne Mamo

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Background: Infection with Brucella melitensis (B. melitensis) is one of the most important causes of abortion in goats and sheep, and also causes severe systemic disease in exposed humans. In Ethiopia, based on seroepidemiological studies, brucellosis is known to be endemic. However, there is little information on the isolation and molecular detection of Brucella species in small ruminants. Therefore, the present study was conducted in the Amibara district of Afar Region of Ethiopia to isolate and molecularly detect Brucella infection in small ruminants. Results: Out of the total 64 samples cultured, eight samples (five vaginal swabs and three milk) were positive for Brucella species based on colony morphology, growth characteristics, modified acid fast staining and biochemical tests results. Further identification using Brucella- ladder PCR method showed that four of the isolates (three from vaginal swabs and one from milk) from goats amplified fragments of 1071 bp, 794 bp, 587 bp, 450 bp and 152 bp in band size. The molecular result combined with the microbiological and biochemical characteristics of the isolates indicated that the isolates were strains of B. melitensis. Conclusion: The finding of this study could suggest economic and zoonotic significance of B. melitensis and warrants for the need for control strategies in livestock and creation of awareness in the pastoral communities on the safe consumption of foods of animal origin and avoidance of physical contact with aborted materials.

Original languageEnglish
Article number108
JournalBMC Microbiology
Volume19
Issue number1
DOIs
Publication statusPublished - May 24 2019
Externally publishedYes

Keywords

  • Abortion
  • Afar region
  • B. melitensis
  • Ethiopia
  • Goats
  • Isolation
  • Molecular detection

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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