Isolation and characterization of plant growth promoting endophytic diazotrophic bacteria from Korean rice cultivars

Sang Hye Ji, Mayank Anand Gururani, Se Chul Chun

Research output: Contribution to journalArticlepeer-review

207 Citations (Scopus)

Abstract

We have isolated 576 endophytic bacteria from the leaves, stems, and roots of 10 rice cultivars and identified 12 of them as diazotrophic bacteria using a specific primer set of nif gene. Through 16S rDNA sequence analysis, nifH genes were confirmed in the two species of Penibacillus, three species of Microbacterium, three Bacillus species, and four species of Klebsiella. Rice seeds treated with these plant growth-promoting bacteria (PGPB) showed improved plant growth, increased height and dry weight and antagonistic effects against fungal pathogens. In addition, auxin and siderophore producing ability, and phosphate solubilizing activity were studied for the possible mechanisms of plant growth promotion. Among 12 isolates tested, 10 strains have shown higher auxin producing activity, 6 isolates were confirmed as strains with high siderophore producing activity while 4 isolates turned out to have high phosphate-solubilizing activity. These results strongly suggest that the endophytic diazotrophic bacteria characterized in this study could be successfully used to promote plant growth and inducing fungal resistance in plants.

Original languageEnglish
Pages (from-to)83-98
Number of pages16
JournalMicrobiological Research
Volume169
Issue number1
DOIs
Publication statusPublished - Jan 20 2014
Externally publishedYes

Keywords

  • Diazotrophic bacteria
  • Endophytic
  • Nitrogen-fixing
  • Plant growth-promoting bacteria (PGPB)
  • Siderophore

ASJC Scopus subject areas

  • Microbiology

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