Insulin receptor tyrosine kinase activity in monocytes of type 2 diabetes mellitus patients receiving oral L-lysine

K. N. Sulochana, M. Rajesh, S. Ramakrishnan

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The action of lysine as an antidiabetic agent was examined in human volunteers. Eight patients with type 2 DM were orally supplemented with L-lysine hydrochloride 1g /day in two doses along with antidiabetic tablets (glyciphage or chlorformine), for a period of two months. Periodically their plasma fasting sugar and insulin receptor tyrosine kinase activity was measured in their monocytes. Eight normal healthy volunteers served as controls for comparison of receptor tyrosine kinase activity. Insulin receptor tyrosine kinase was isolated from monocytes by immunoprecipitation and the activity was determined using exogenous substrate poly glu-tyr (4:1) and radioactive ATP. Phosphorylated peptide was separated by electrophoresis and quantified using a liquid scintillation system. The enzyme activity was significantly low (22074±1728 dpm/ml immunoprecipitate) in subjects with diabetes when compared to non-diabetic control group (50,775 ± 3597). Lysine treatment enhanced the enzyme activity by 31% in patients with diabetes and decreased their blood sugar by 27%.

Original languageEnglish
Pages (from-to)331-334
Number of pages4
JournalIndian Journal of Biochemistry and Biophysics
Volume38
Issue number5
Publication statusPublished - Oct 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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