Inhibition of cromakalim-activated K+ current by ethanol in follicle-enclosed Xenopus oocytes

Murat Oz, Yulia Tchugunova, Meral Dinc

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Ethanol has been reported to modulate arterial dilation and insulin secretion. ATP-inhibited K+ channels (KATP) are reported to have regulatory roles during these events. In the present study, the effect of ethanol on K+ currents activated by the KATP channel opener cromakalim was investigated in follicular cells of Xenopus oocytes. The results indicate that ethanol in the concentration range of 10-300 mM (approximately 0.06%-2% v/v) reversibly inhibits the cromakalim-induced K+ currents. The 50% of maximal ethanol effect was obtained at 53 mM. Inhibition of cromakalim-activated K+ current by ethanol was non-competitive. In oocytes treated with the Ca2+-chelator BAPTA, inhibition of cromakalim-induced K+ currents by ethanol was not altered, suggesting that Ca2+-activated second messenger pathways were not involved in the actions of ethanol. Similarly, currents activated by 8-Br-cAMP were also inhibited by ethanol, but the ethanol inhibition of 8-Br-cAMP-activated currents was significantly less than inhibition of cromakalim-activated currents by ethanol. These results indicate that cromakalim-activated K+ currents in follicular cells of Xenopus oocytes were modulated by ethanol.

Original languageEnglish
Pages (from-to)80-85
Number of pages6
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume367
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

Keywords

  • Cromakalim
  • Ethanol
  • K channels
  • Xenopus oocytes

ASJC Scopus subject areas

  • Pharmacology

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