Identification of proteins present in the Douglas fir ovular secretion: An insight into conifer pollen selection and development

Brett A.D. Poulis, Stephen J.B. O'Leary, Jody D. Haddow, Patrick Von Aderkas

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

A week before fertilization in Douglas fir, a secretion fills the micropylar chamber of the ovule that houses the engulfed pollen. This liquid initiates pollen tube formation. This secretion is rich in proteins. Proteomic analysis using gel electrophoresis, combined with quadrupole time-of-flight tandem mass spectrometry peptide sequencing, identified nine of the more abundant proteins as a 90 kDa xylosidase with an isoelectric point (pI) of 6.6, a 65 kDa xylosidase with a pI of 6.0, a 70 kDa invertase with a pI of 6.3, a 50 kDa invertase with a pI of 6.5, a 45 kDa galactosidase with a pI of 7.8, a 29 kDa galactosidase with a pI of 5.9, a 40 kDa aspartyl protease with a pI of 5.5, a 37 kDa peroxidase with a pI of 7.9, and a 33 kDa serine carboxypeptidase-like protein with a pI of 4.5. This research presents the first evidence that conifer ovular secretion proteins may influence pollen selection and development.

Original languageEnglish
Pages (from-to)733-739
Number of pages7
JournalInternational Journal of Plant Sciences
Volume166
Issue number5
DOIs
Publication statusPublished - Sep 2005

Keywords

  • Conifers
  • Ovular secretion
  • Pollen development
  • Pollen selection

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

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