Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora

J. Michael Conlon, Agnes Sonnevend, Carlos Davidson, Anni Demandt, Thierry Jouenne

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Antimicrobial peptides in the skin secretions of anurans constitute a component of the innate immunity that protects the organism against invading pathogens. Four peptides with antimicrobial activity were isolated in high yield from norepinephrine-stimulated skin secretions of the Northern red-legged frog Rana aurora aurora and their primary structures determined. Ranatuerin-2AUa (GILSSFKGVAKGVAKNLAGKLLDELKCKITGC) showed potent growth-inhibitory activity against a range of Gram-positive and Gram-negative bacteria (minimum inhibitory concentrations <20 μM) but low hemolytic activity against human erythrocytes (50% hemolysis at 290 μM). Brevinin-1AUa (FLPILAGLAAKLVPKVFCSITKKC) and brevinin-1AUb (FLPILAGLAANILPKVFCSITKKC) also showed potent antimicrobial activity but were strongly hemolytic (HC 50<10 μM). Temporin-1AUa (FLPIIGQLLSGLL.NH2) atypically lacked a basic amino acid residue and showed very weak antimicrobial and hemolytic activity. Its biological function remains to be established. The primary structures of the antimicrobial peptides are consistent with a close phylogenetic relationship between R. aurora, Rana boylii and Rana luteiventris.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalDevelopmental and Comparative Immunology
Volume29
Issue number1
DOIs
Publication statusPublished - 2005
Externally publishedYes

Keywords

  • Antimicrobial peptide
  • Brevinin-1
  • Frog skin
  • HC, concentration producing 50% hemolysis
  • MIC, minimum inhibitory concentration
  • Phylogeny
  • Ranatuerin-2
  • Temporin

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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