Histone acetyltransferase complexes stabilize SWI/SNF binding to promoter nucleosomes

Ahmed H. Hassan, Kristen E. Neely, Jerry L. Workman

Research output: Contribution to journalArticlepeer-review

298 Citations (Scopus)

Abstract

To investigate the function of SWI/SNF in site-specific chromatin remodeling at promoters, we have used a purified system to analyze its distribution, function, and retention following recruitment by a sequence-specific transcription activator. Activator recruitment of SWI/SNF bound the complex to promoter proximal nucleosomes and led to localized nucleosome disruption. However, retention of SWI/SNF on the promoter required either the continued binding of the transcription activator or acetylated histones. Histone acetylation by either the SAGA or NuA4 HAT complexes increased the retention of SWI/SNF on the promoter. These data illustrate a functional link between HAT complexes and the SWI/SNF chromatin remodeling complex and provide a mechanistic basis for the ordered recruitment of these complexes.

Original languageEnglish
Pages (from-to)817-827
Number of pages11
JournalCell
Volume104
Issue number6
DOIs
Publication statusPublished - Mar 23 2001
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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