High-performance liquid chromatographic analysis of secoisolariciresinol diglucoside and hydroxycinnamic acid glucosides in flaxseed by alkaline extraction

Christina Eliasson, Afaf Kamal-Eldin, Roger Andersson, Per Åman

Research output: Contribution to journalArticlepeer-review

130 Citations (Scopus)

Abstract

A HPLC method was developed for the analysis of secoisolariciresinol diglucoside (SDG) and hydroxycinnamic acid glucosides in milled defatted flaxseed flour. Direct extraction by 1 M NaOH for 1 h at 20°C resulted in a higher yield than that obtained by hydrolysis of alcoholic extracts. An internal standard, o-coumaric acid, was used and the method was found to be easy, fast, and with good repeatability. On dry matter basis, different samples of flaxseeds varied considerably in their content of (+)-SDG (11.9-25.9 mg/g), (-)-SDG (2.2-5.0 mg/g), p-coumaric acid glucoside (1.2-8.5 mg/g), and ferulic acid glucoside (1.6-5.0 mg/g).

Original languageEnglish
Pages (from-to)151-159
Number of pages9
JournalJournal of Chromatography A
Volume1012
Issue number2
DOIs
Publication statusPublished - Sep 19 2003
Externally publishedYes

Keywords

  • Coumaric acid glucoside
  • Ferulic acid glucoside
  • Glucosides
  • Hydroxycinnamic acid glucosides
  • Lignans
  • Linum usitatissium
  • Plant materials
  • Secoisolariciresinol diglucoside

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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