Growth inhibition of MCF-7 human breast cancer cells by progesterone is associated with cell differentiation and phosphorylation of Akt protein

M. Alkhalaf, A. El-Mowafy, S. Karam

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Progesterone inhibits the proliferation of normal breast epithelial cells in vivo as well as breast cancer cells in vitro. The molecular mechanisms of this inhibition are not fully understood. The purpose of this study was to investigate the capacity of progesterone to induce apoptosis and to alter the activity of a key regulator of cell growth and differentiation, the Akt protein. We show here that (i) growth inhibition of breast cancer cells by progesterone is due to the induction of cell differentiation and not to apoptosis; (ii) progesterone activates the PI3-kinase/Akt pathway as shown by the increase in the phosphorylation of Akt protein; (iii) inhibiting PI3-kinase/Akt pathway with LY294002 causes stimulation of apoptosis; and (v) progesterone enhances LY294002 induced-growth inhibition and apoptosis. These results suggest that progesterone may protect breast cancer cells from apoptosis by altering PI3-kinase activity and that MCF-7 cells become more sensitive to progesterone and die by apoptosis upon inhibition of the PI3-kinase/Akt pathway.

Original languageEnglish
Pages (from-to)481-488
Number of pages8
JournalEuropean Journal of Cancer Prevention
Volume11
Issue number5
DOIs
Publication statusPublished - Oct 2002
Externally publishedYes

Keywords

  • Akt
  • Apoptosis
  • Breast cancer
  • MCF-7
  • PI3 K
  • Progesterone

ASJC Scopus subject areas

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

Fingerprint

Dive into the research topics of 'Growth inhibition of MCF-7 human breast cancer cells by progesterone is associated with cell differentiation and phosphorylation of Akt protein'. Together they form a unique fingerprint.

Cite this