Growth and Differentiation of Dental Stem Cells of Apical Papilla on Polycaprolactone Scaffolds

Mohamed Jamal, Yaser Greish, Sami Chogle, Harold Goodis, Sherif M. Karam

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Biodegradable scaffolds are useful tools in the field of tissue engineering and regenerative medicine. The aim of this study was to test the potential of the human stem cells of apical papilla (SCAP) to attach, proliferate and differentiate on a polycaprolactone (PCL)-based scaffolds. SCAP were extracted from the root apical papillae of freshly extracted immature premolar teeth by using enzymatic digestion. Porous PCL scaffolds were fabricated using particle leaching method and NaCl or mannitol as porogens. SCAP of passage 3 were seeded on non-porous and porous PCL scaffolds for up to 14 days. For control, cells were cultured on glass coverslips. Picogreen DNA quantification was used to assay for cell proliferation. Cell differentiation and development of calcification nodules were examined using scanning electron microscopy and alizarin red staining. SCAP showed a comparable attachment, growth and proliferation patterns on PCL scaffolds and coverslips. Cell proliferation was enhanced on mannitol scaffolds at all time points. Calcification nodules were detected in all PCL scaffolds while it was not present on glass coverslips. These nodules were detected on NaCl-scaffolds by day 7 and on mannitol and non-porous scaffolds by day 14. In conclusion, SCAP were able to attach, proliferate and differentiate on PCL scaffolds without using any inductive media, indicating their potential application for dental tissue regeneration.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages31-40
Number of pages10
DOIs
Publication statusPublished - 2018

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1077
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • Calcification nodules
  • Dental stem cells
  • Polycaprolactone
  • Porous scaffolds
  • Regenerative endodontics
  • Stem cells of apical papilla

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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