Glial fibrillary acidic protein (GFAP) from goldfish: Its localisation in visual pathway

S. N. Nona, S. A.S. Shehab, C. A. Stafford, J. R. Cronly‐Dillon

Research output: Contribution to journalArticlepeer-review

85 Citations (Scopus)

Abstract

An intermediate filament fraction, isolated from goldfish brain, contains a prominent protein having a molecular weight of 51 kDa. In normal goldfish visual pathway, this protein is present in tectum and tract, but not in optic nerve. A polyclonal antibody raised to this protein clearly labels ependymal glial profiles in tectum and parallel processes in the tract, whereas optic nerve is unlabelled; Müller fibres in the retina are also labelled. A similar, but less prominent, pattern of staining is observed with antibodies, raised elsewhere, against glial fibrillary acidic protein from human and porcine. These results suggest that the 51 kDa protein is a GFAP, demonstrate the heterogeneity of astrocytes in goldfish visual pathway, and are consistent with the idea that GFAP is well conserved in vertebrate phylogeny.

Original languageEnglish
Pages (from-to)189-200
Number of pages12
JournalGlia
Volume2
Issue number3
DOIs
Publication statusPublished - 1989
Externally publishedYes

Keywords

  • Astrocytes
  • Ependymal glia
  • Gel electrophoresis
  • Immunohistochemistry
  • Intermediate filaments

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

Fingerprint

Dive into the research topics of 'Glial fibrillary acidic protein (GFAP) from goldfish: Its localisation in visual pathway'. Together they form a unique fingerprint.

Cite this